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KMID : 0380020180330020083
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Korean Journal of Biotechnology and Bioengineering
2018 Volume.33 No. 2 p.83 ~ p.88
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Effect of Medium Composition on Cell Growth and Product Formation in Clostridium autoethanogenum Culture
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Ahn Bo-Hye
Park So-Eun
Kim Young-Kee
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Abstract
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In this work, optimization of the culture medium in the fermentation process of Clostridium autoethanogenum, an acetogenic microorganism, to produce ethanol from synthesis gas, was examined to improve the ethanol production. The components of culture medium, such as fructose, NH4Cl, and K2HPO4, were selected as influence factors for cell growth and ethanol production. As the concentration of fructose increased, the cell mass increased from 0.178 to 1.069 at yeast extract of 0.5 g/L and the cell mass increased from 0.715 to 2.260 at yeast extract of 5 g/L. But the ethanol production decreased when the concentration of fructose was higher than that of base medium (5 g/L). And the specific ethanol productivity was the highest when fructose was not used. Cell growth and ethanol production were not significantly influenced by NH4Cl concentration, however growth inhibition was observed at 30 g/L of NH4Cl. As the concentration of K2HPO4 increased, both of cell growth and ethanol production increased. From experiments with NH4Cl and K2HPO4, specific ethanol productivities were higher when low quantity of yeast extract (0.5 g/L) was used.
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KEYWORD
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acetogen, bioethanol, culture medium, optimization, Clostridium autoethanogenum
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